Optimization of the process of lyophilization of microbial culture of bacillus subtilis nbimcc 2353 by ultrasound

Lyophilization is a process in which water transforms from a solid to a gaseous aggregate state in a medium of vacuum and low temperature. In this type of processing of natural extracts, the biologically active substances in their composition remain unchanged. The continuous processing (24 - 72 hours), including freezing and drying in vacuum conditions and temperature regime from - 30℃ to + 30℃, is associated with a high energy consumption, which is a disadvantage of the method. In the present publication, the authors present experimental data on the possibilities of using ultrasonic waves during lyophilization in order to reduce the process duration, production costs and to achieve low costs of the process. The subject of the research is a microbial culture, obtained from a producer strain of Bacillus subtilis NBIMCC 2353. It has been recorded that the use of an ultrasonic wave with a frequency of 40 kHz reduces the processing time by 32% which reduces the cost of the process, while maintaining high survival rates of the microbial cells. Mention the abstract for the article. An abstract is a brief summary of a research article, thesis, review, conference proceeding or any in-depth analysis of a particular subject or discipline, and is often used to help the reader quickly ascertain the paper's purpose. When used, an abstract always appears at the beginning of a manuscript, acting as the point-of-entry for any given scientific paper or patent application.