Invitro antibacterial activity and phytochemical profile analysis by tlc and gcms from the chloroform extract of chrozophora rottleri

The aim of this study was to bearing the phytochemical profiles from the chloroform extract of Chrozophora rottleri leaves and it’s evaluate antibacterial activity. The chloroform extract of Chrozophora rottleri leaves were prepared by ethanol gradient elution orderly and analyzed by TLC and GCMS. The bacterial strains were used to evaluate the antibacterial activities by the disc diffusion and MIC method. Results showed that the disc diffusion against bacteria ranged from 5 µL/mL to 20 µL/mL of Escherichia coli, Staphylococcus aureus and Proteus vulgaris). Also chloroform extract exhibited MIC values ranging 5 µL/mL against both gram positive and negative bacteria. Remarkable antibacterial potential was noticeable with higher inhibition zone recorded in Escherichia coli, Staphylococcus aureus than other organism. The TLC fingerprint profiles demonstrated the presence of various phyto chemicals in leaf extract. The GCMS analysis revealed quercetin and kaempferol as major ingredients in the screening chloroform extract of Ch. rottleri. Interestingly, the content of quercetin and kaempferol varied with the concentration of elution ethanol. In conclusion, the chloroform extract of Ch. Rottleri possessed the property like antibiotics against bacteria. These results support an individual phytochemical profile further investigation for the isolation of novel compounds with antimicrobial bioactivity and also afford hypothetical supporting as natural food preservatives and medicinal plant.