16s Rrna gene sequence, secondary structure and restriction sites analysis of isolated actinomycetes from soil samples of botanical garden, Karnatak University, Dharwad
International Journal of Development Research
16s Rrna gene sequence, secondary structure and restriction sites analysis of isolated actinomycetes from soil samples of botanical garden, Karnatak University, Dharwad
Received 24th May, 2017; Received in revised form 15th June, 2017; Accepted 23rd July, 2017; Published online 30th August, 2017
Copyright ©2017, Sreenivasa Nayaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current researchers are more emphasised towards microbes to meet the antibiotic requirement of mankind. And in this study we made an attempt for the search of antibiotics through isolation of actinomycetes and analysis of their antibiocity through 16s rRNA gene sequencing and identification of restriction site. An Actinomycete species (SN-4) was isolated from botanical garden soil samples of the Karnatak University, Dharwad, India. Morphological, physiological, biochemical and 16S ribosomal RNA studies suggested that the isolate belongs to Streptomyces sp. Preliminary screening of antimicrobial activity revealed that the SN-4 isolate was active against all the bacterial pathogens and can be used as source of antibiotics against various diseases causing pathogens. The rRNA secondary structure and the restriction sites of Streptomyces sp. SN-4 was predicted using Gene Bee and NEB Cutter online tools.
